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A comparative ultrastructural study of pit membranes with plasmodesmata associated thickenings in four angiosperm species Naturalis
Rabaey, D.; Lens, F.; Huysmans, S.; Smets, E.; Jansen, S..
Recent micromorphological observations of angiosperm pit membranes have extended the number and range of taxa with pseudo-tori in tracheary elements. This study investigates at ultrastructural level (TEM) the development of pseudo-tori in the unrelated Malus yunnanensis, Ligustrum vulgare, Pittosporum tenuifolium, and Vaccinium myrtillus in order to determine whether these plasmodesmata associated thickenings have a similar developmental pattern across flowering plants. At early ontogenetic stages, the formation of a primary thickening was observed, resulting from swelling of the pit membrane in fibre-tracheids and vesselelements. Since plasmodesmata appear to be frequently, but not always, associated with these primary pit membrane thickenings, it remains...
Tipo: Article / Letter to the editor Palavras-chave: Ontogeny; Pseudo-torus; Tracheary element; Pit membrane; Bordered pit; Wood ultrastructure; 42.56.
Ano: 2008 URL: http://www.repository.naturalis.nl/record/430078
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A comparison of paraffin and resin-based techniques used in bark anatomy Naturalis
Hamann, T.; Smets, E.; Lens, F..
Bark anatomy is an unappreciated discipline in plant systematics, despite its great potential to reveal systematically informative features. In this paper, main reasons for the lack of detailed bark anatomical data in many plant families are identified, including problems with sectioning, terminological issues, and difficulties in observation of dilated stems. We deal with these problems by focusing on two aspects: (1) compare, discuss and improve existing sectioning and maceration techniques using two species with soft and hard bark tissues; and (2) discuss the best way to collect stem bark samples. We hope that this paper will stimulate inclusion of bark anatomical data in future systematic studies.
Tipo: Article / Letter to the editor Palavras-chave: Bark anatomy; Light microscopy; Macerations; Plant systematics; Phloem; Sectioning; 42.54.
Ano: 2011 URL: http://www.repository.naturalis.nl/record/421834
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A histological study of microsporogenesis in Tarenna gracilipes (Rubiaceae) Naturalis
Vinckier, S.; Smets, E..
We studied the microsporogenesis in Tarenna gracilipes (Hayata) Ohwi, with special attention to the mode of exine deposition and tapetum development. We based this research on light (LM), scanning electron (SEM) and transmission electron microscopic (TEM) observations of developing anthers of T. gracilipes, from the microspore mother cell stage towards anther dehiscence. Evidence is supplied that the microsporogenesis in T. gracilipes can be considered as simultaneous. Columellae, foot layer and tectum develop in a fibrillar matrix. Similar with earlier studies in Rubiaceae species, a single white line formed near the plasmalemma in the extra-apertural region. The developing endexine dilated into several white line centered lamellae at the apertures. An...
Tipo: Article / Letter to the editor Palavras-chave: Tarenna gracilipes; Rubiaceae; Microsporogenesis; Exine deposition; Tapetum development; Histology; Light microscopy; Scanning.
Ano: 2005 URL: http://www.repository.naturalis.nl/record/407305
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A New Heterophyllous Spermacoce Species (Rubiaceae) from the Marungu Highlands, Democratic Republic of the Congo Naturalis
Dessein, S.; Robbrecht, E.; Smets, E..
Spermacoce heteromorpha Dessein (Rubiaceae, Spermacoceae) is newly described and illustrated. It grows on the Marungu highlands of the Democratic Republic of the Congo (D.R. Congo) at altitudes of 1800 to 1900 m. The species resembles the Tanzanian Spermacoce taylorii Verdcourt, from which it mainly differs in having terminal inflorescences, only slightly exserted anthers, and densely pubescent stems. Moreover, Spermacoce heteromorpha features marked heterophylly, with long leaves at the plant’s base and much shorter cauline leaves, a type of heterophylly rarely observed in Spermacoce or in the Rubiaceae as a whole. The finding of this species exemplifies the unique floristic richness of this underexplored part of D.R. Congo.
Tipo: Article / Letter to the editor Palavras-chave: Democratic Republic of the Congo; Heterophylly; Marungu highlands; Rubiaceae; Spermacoce.
Ano: 2006 URL: http://www.repository.naturalis.nl/record/407334
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A Plastid Gene Phylogeny of the Yam Genus, Dioscorea: Roots, Fruits and Madagascar Naturalis
Wilkin, P.; Schols, P.; Chase, M.; Chayamarit, K.; Furness, C.; Huysmans, S.; Rakotonasolo, F.; Smets, E.; Thapyai, C..
Following recent phylogenetic studies of the families and genera of Dioscoreales, the identification of monophyletic infrageneric taxa in the pantropical genus Dioscorea is a priority. A phylogenetic analysis based on sequence data from the plastid genes rbcL and matK is presented, using 67 species of Dioscorea and covering all the main Old World and selected New World lineages. The analysis used 14 outgroup taxa, including Trichopus Gaertn., Tacca J.R. & G. Forster, Stenomeris Planch., Burmannia L. and Thismia Griff. The main findings are: a) that a clade of rhizomatous taxa is sister to the rest of Dioscorea; b) the main Old World groups (such as the right-twining D. sect. Enantiophyllum) are monophyletic and c) there are two distinct lineages among...
Tipo: Article / Letter to the editor Palavras-chave: Dioscorea; Phylogeny; Classification; Analysis; Plastid genes; RbcL; MatK; Character evolution.
Ano: 2005 URL: http://www.repository.naturalis.nl/record/407311
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A search for phylogenetically informative wood characters within Lecythidaceae s.l. Naturalis
Lens, F.; Baas, P.; Jansen, S.; Smets, E..
The wood structure of 71 species representing 24 genera of the pantropical Lecythidaceae s.l., including the edible Brazil nuts (Bertholletia excelsa) and the spectacular cannon-ball tree (Couroupita guianensis), was investigated using light and scanning electron microscopy. This study focused on finding phylogenetically informative characters to help elucidate any obscure evolutionary patterns within the family. The earliest diverging subfamily Napoleonaeoideae has mixed simple/scalariform vessel perforations, scalariform vessel-ray pitting, and high multiseriate rays, all features that are also present in Scytopetaloideae. The wood structure of Napoleonaea is distinct, but its supposed close relative Crateranthus strongly resembles Scytopetaloideae. The...
Tipo: Article / Letter to the editor Palavras-chave: Ericales; Lecythidaceae s.l.; Lecythidaceae s.s.; Napoleonaeaceae; Scytopetalaceae; Systematic wood anatomy; 42.48.
Ano: 2007 URL: http://www.repository.naturalis.nl/record/422216
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Are the genera Hallea and Mitragyna (Rubiaceae-Coptosapelteae) pollen morphologically distinct? Naturalis
Huysmans, S.; Robbrecht, E.; Smets, E..
Recent literature is controversial as regards the segregation of Hallea and Mitragyna, and pretends that the two genera show pollen morphological differences. In the present study the pollen morphology of all ten species of the complex is described on the basis of light and scanning electron microscopy (including examination of broken grains, which were obtained with a technique never applied in palynology, viz. shaking with glass beads). The two genera have 3-zonocolporate grains with compound apertures (endoapertures are always H-shaped, sometimes incompletely so). While Hallea showed to be stenopalynous (sexine always tectate-perforate), Mitragyna is more variable (sexine microreticulate or tectate-perforate), and several of its species have pollen...
Tipo: Article / Letter to the editor
Ano: 1994 URL: http://www.repository.naturalis.nl/record/524503
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Comparative Pollen Development in Dioscoreales Naturalis
Schols, P.; Furness, C.; Merckx, V.; Wilkin, P.; Smets, E..
Pollen and orbicule ontogeny in representatives of three genera of Dioscoreales—Narthecium ossifragum Huds. (Nartheciaceae), Tacca artocarpifolia Seem. and Tacca chantrieri Andre´ (Dioscoreaceae), and Dioscorea communis (L.) Caddick & Wilkin (Dioscoreaceae)—is described and illustrated using LM, SEM, and TEM. The main difference is in microsporogenesis, which is successive in Narthecium Huds. and simultaneous in Tacca J.R. & G. Forst. and Dioscorea L. This is reflected in the tetrad configuration but not in the apertures: Narthecium and Tacca are monosulcate, while Dioscorea has two equatorial apertures. Other features of pollen development are similar in all three genera. Exine development starts at the proximal pole, and during the tetrad stage,...
Tipo: Article / Letter to the editor Palavras-chave: Intine; Microsporogenesis; Monocotyledons; Orbicules; Pollen ontogeny; Systematics; Tapetum; Tetrads.
Ano: 2005 URL: http://www.repository.naturalis.nl/record/407299
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Comparative wood anatomy of Andromedeae s.s., Gaultherieae, Lyonieae and Oxydendreae (Vaccinioideae, Ericaceae s.l.) Naturalis
Lens, F.; Smets, E.; Jansen, S..
The wood anatomical structure of 11 out of 13 genera from four tribes of the Vaccinioideae, namely Andromedeae s.s., Gaultherieae, Lyonieae and Oxydendreae (Ericaceae s.l.), is described using light and scanning electron microscopy. Several features of the secondary xylem support the tribal classification based on molecular data: arrangement of vessel-ray pitting, height of multiseriate rays and the shape of the body ray cells. Oxydendreae are clearly defined from the other representatives by various wood anatomical features. Gaultherieae can be distinguished from Lyonieae by differences in vessel perforation plates, vessel-ray pitting, height and structure of multiseriate rays, and occurrence of prismatic crystals, but the wood of Andromedeae s.s. is...
Tipo: Article / Letter to the editor Palavras-chave: Comparative wood anatomy; Andromedeae; Gaultherieae; Lyonieae; Oxydendreae; Vaccinioideae; Ericaceae; Andromedeae sensu Stevens; Pith structure; 42.58.
Ano: 2004 URL: http://www.repository.naturalis.nl/record/424620
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Comparative wood anatomy of epacrids (Styphelioideae, Ericaceae s.l.) Naturalis
Lens, F.; Gasson, P.; Smets, E.; Jansen, S..
The wood anatomy of 16 of the 37 genera within the epacrids (Styphelioideae, Ericaceae s.l.) is investigated by light and scanning electron microscopy. Several features in the secondary xylem occur consistently at the tribal level: arrangement of vessel‐ray pits, distribution of axial parenchyma, ray width, and the presence and location of crystals. The primitive nature of Prionoteae and Archerieae is supported by the presence of scalariform perforation plates with many bars and scalariform to opposite vessel pitting. The wood structure of Oligarrheneae is similar to that of Styphelieae, but the very narrow vessel elements, exclusively uniseriate rays and the lack of prismatic crystals in Oligarrheneae distinguish these two tribes. The secondary xylem of...
Tipo: Article / Letter to the editor Palavras-chave: Ecological wood anatomy; Epacrids; Ericaceae s.l.; Styphelioideae; Systematic wood anatomy; 42.56.
Ano: 2003 URL: http://www.repository.naturalis.nl/record/431740
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Comparative wood anatomy of the blueberry tribe (Vaccinieae, Ericaceae s.l.) Naturalis
Lens, F.; Kron, K.A.; Luteyn, J.L.; Smets, E.; Jansen, S..
Wood samples of 111 Vaccinieae specimens (Vaccinioideae, Ericaceae s.l.) representing 98 species and 26 genera are investigated with light microscopy and scanning electron microscopy. The wood structure of Vaccinieae delivers taxonomically important characters that can be used to define some subclades within the tribe. The wood of the large polyphyletic genus Vaccinium strongly resembles non-vaccinioid members of the family, which are characterized by bordered vessel-ray pits and relatively narrow (2- to 4-seriate) and low multiseriate rays (often less than 1000 mm) with exclusively or mainly procumbent body ray cells. The East Malesian clade, Meso-American/Caribbean clade, and the Andean clade show a combination of wood anatomical features that is lacking...
Tipo: Article / Letter to the editor Palavras-chave: Blueberries; Comparative wood anatomy; Ericaceae; Neotropics; Secretory ducts; Systematics; Vaccinieae; 42.56.
Ano: 2004 URL: http://www.repository.naturalis.nl/record/423810
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Comparative Wood Anatomy of the Primuloid Clade (Ericales s.l.) Naturalis
Lens, F.; Jansen, S.; Caris, P.; Serlet, L.; Smets, E..
The wood structure of 78 species from 27 genera representing the woody primuloids (Maesaceae, Myrsinaceae, and Theophrastaceae) was investigated using light microscopy (LM) and scanning electron microscopy (SEM). Results indicated that the ray structure, the nature of mineral inclusions, and the occurrence of breakdown areas in rays can be used to separate the three primuloid families from each other. Within Ericales, the presence of exclusively multiseriate rays is synapomorphic for Myrsinaceae and Theophrastaceae, and the occurrence of breakdown areas in rays is synapomorphic for Myrsinaceae. Within Myrsinaceae, the wood structure of the mangrove genus Aegiceras differs because it has short vessel elements that are storied, non-septate fibers, a...
Tipo: Article / Letter to the editor Palavras-chave: Primuloids; Ericales s.l.; Maesaceae; Myrsinaceae; Theophrastaceae; Wood anatomy; Comparison; Light microscopy; Scanning electron microscopy.
Ano: 2005 URL: http://www.repository.naturalis.nl/record/407307
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Contributions to the Wood Anatomy of the Rubioideae (Rubiaceae) Naturalis
Jansen, S.; Lens, F.; Ntore, S.; Piesschaert, F.; Robbrechf, E.; Smets, E..
The secondary xylem of Craterispermeae, Coussareeae, Morindeae s.str., Prismatomerideae, Pauridiantheae, Urophylleae, and Triainolepideae (Rubiaceae, Rubioideae) is described and illustrated in detail. Genera that were previously placed in the Morindeae or Psychotrieae such as Damnacanthus, Lasianthus, Saldinia, and Trichosfachys are also included. Wood anatomical characters are compared with recent phylogenetic insights into the study group on the basis of molecular data. The observations demonstrate that the delimitation and separation of several taxa from the former Coussareeae/Morindeae/Prismatomerideae/Psychotrieae aggregate is supported by wood anatomical data. The Coussareeae can be distinguished from the other Rubioideae by their scanty parenchyma,...
Tipo: Article / Letter to the editor Palavras-chave: Rubiaceae; Rubioideae; Systematic wood anatomy; 42.58.
Ano: 2001 URL: http://www.repository.naturalis.nl/record/421859
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Description of 11 new Astiella (Spermacoceae, Rubiaceae) species endemic to Madagascar Naturalis
Groeninckx, I.; Janssens, S.; Smets, E.; Verstraete, B..
Astiella is an herbaceous genus endemic to Madagascar, originally described with a single species A. delicatula Jovet. Molecular and morphological evidence place it in the tribe Spermacoceae s. lat. of Rubiaceae. During herbarium studies and fieldwork in Madagascar, 11 new Astiella species were identified and these are described here: A. antongilensis Groeninckx sp. nov., A. antsalovansis Groeninckx sp. nov., A. confusa Groeninckx sp. nov., A. deblockiae Groeninckx sp. nov., A. desseinii Groeninckx sp. nov., A. homolleae Groeninckx sp. nov., A. latifolia Groeninckx sp. nov., A. longifimbria Groeninckx sp. nov., A. perrieri Groeninckx sp. nov., A. pulla Groeninckx sp. nov., and A. tsaratanensis Groeninckx sp. nov. The genus Astiella now holds 12 species in...
Tipo: Article / Letter to the editor Palavras-chave: Astiella; Endemism; Madagascar; Rubiaceae; Spermacoceae s. lat..
Ano: 2017 URL: http://www.repository.naturalis.nl/record/629097
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Ecological trends in the wood anatomy of Vaccinioideae (Ericaceae s.l.) Naturalis
Lens, F.; Luteyn, J.L.; Smets, E.; Jansen, S..
The ecological wood anatomy of 128 vaccinioid wood samples (including 115 species, 35 genera), collected between 39°S and 60°N latitude and 10 m to 3400 m altitude is studied. Several wood anatomical features within the subfamily, viz. tangential vessel diameter, average length of tracheary elements, height of multiseriate rays, and presence of prismatic crystals are negatively correlated with increasing latitude, while vessel density and helical thickenings show a positive correlation with increasing latitude. Similar latitudinal trends are found within the genus Vaccinium (31 species studied). The correlation between various wood anatomical features and latitude is surprisingly high despite the fact that most tropical species grow in montane regions,...
Tipo: Article / Letter to the editor Palavras-chave: Altitude; Ecological and functional wood anatomy; Ericaceae; Latitude; Vaccinioideae; Vaccinium; 42.58.
Ano: 2004 URL: http://www.repository.naturalis.nl/record/424624
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Elaborate Petals in Australian Spermacoce (Rubiaceae) Species: Morphology, Ontogeny and Function Naturalis
Vaes, E.; Vrijdaghs, A.; Smets, E.; Dessein, S..
Background and Aims Australian Spermacoce species display various types of elaborate petals. Their precise morphology, ontogenetic origin, and function are hitherto unknown. The aim of the present paper is to unravel the development and nature of the diverse types of elaborate petals in Spermacoce through a floral ontogenetic study. Methods The floral ontogeny of six species characterized by different types of corolla appendages was studied by scanning electron microscopy and light microscopy. In order to elucidate the possible functions of the elaborate petals, field observations were conducted as well. Key Results Scanning electronmicrographs show that full-grown petals of Spermacoce lignosa, S. phaeosperma and S. redacta bear appendages on their...
Tipo: Article / Letter to the editor Palavras-chave: Australia; Corolla appendages; Elaborate petals; Floral ontogeny; Pollination; Rubiaceae; Spermacoce brevidens; S. caudata; S. erectiloba; S. lignosa; S. phaeosperma; S. redacta.
Ano: 2006 URL: http://www.repository.naturalis.nl/record/407325
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Evolution of fruit and seed characters in the Diervilla and Lonicera clades (Caprifoliaceae, Dipsacales) Naturalis
Jacobs, B.; Lens, F.; Smets, E..
Background and Aims: The Diervilla and Lonicera clades are members of the family Caprifoliaceae (Dipsacales sensu Donoghue et al., 2001, Harvard Papers in Botany 6: 459–479). So far, the intergeneric relationships of the Lonicera clade and the systematic position of Heptacodium remain equivocal. By studying fruit and seed morphology and anatomy, an attempt is made to clarify these issues. In addition, this study deals with the evolution of fruit and seed characters of the Diervilla and Lonicera clades with reference to allied taxa. Methods: Light and scanning electron microscopy were used for the morphological and anatomical investigations. Phylogenetic analyses were carried out by applying the parsimony and Bayesian inference optimality criteria....
Tipo: Article / Letter to the editor Palavras-chave: Diervilla; Weigela; Symphoricarpos; Lonicera; Triosteum; Leycesteria; Heptacodium; Caprifoliaceae; Dipsacales; Fruit; Seed; Evolution; 42.56.
Ano: 2009 URL: http://www.repository.naturalis.nl/record/421854
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Floral development in three species of Impatiens (Balsaminaceae) Naturalis
Caris, P.L.; Geuten, K.P.; Janssens, S.B.; Smets, E..
The floral morphological and developmental patterns in three species of Impatiens (Balsaminaceae), namely I. columbaria, I. hawkeri, and I. niamniamensis, were studied to contribute to a better understanding of floral evolution in the genus. Strangely enough, the highly diverse floral morphology and ontogeny of this horticulturally important genus have never been studied thoroughly (e.g., using scanning electron microscopic techniques). We discuss the position and the developmental sequence of the different perianth members. We hypothesized that in the course of evolution, the anterolateral sepals become reduced and that a morphocline can be recognized going from species with five sepals, over species with rudimentary sepals that fuse postgenitally with...
Tipo: Article / Letter to the editor Palavras-chave: Balsaminaceae; Ericales; Floral development; Floral evolution; Floral morphology; Impatiens; Scanning electron microscopy; 42.40.
Ano: 2006 URL: http://www.repository.naturalis.nl/record/422332
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Floral Ontogeny in Ficinia and Isolepis (Cyperaceae), with Focus on the Nature and Origin of the Gynophore Naturalis
Vrijdaghs, H.; Goetghebeur, P.; Muasya, A.M.; Caris, P.; Smets, E..
! Background and Aims The generic delimitations of Ficinia and Isolepis, sister genera in the Cypereae, are blurred. Typical Ficinia flowers have a lobed gynophore, which envelops the base of the nutlet, whereas in Isolepis the character is considered to be absent. Some former species of Isolepis, lacking the gynophore, were recently included in Ficinia. The floral ontogeny of representative taxa in Ficinia and Isolepis were investigated with the aim of evaluating the origin and nature of the gynophore in the Cypereae. ! Methods The spikelet and floral ontogeny in inflorescences collected in the field was investigated using scanning electron microscopy (SEM) and light microscopy (LM). ! Key Results SEM images of Isolepis setacea and I. antarctica, Ficinia...
Tipo: Article / Letter to the editor Palavras-chave: Ficinia; Floral ontogeny; Gynophore; Isolepis; Scanning electron microscopy.
Ano: 2005 URL: http://www.repository.naturalis.nl/record/407286
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Floral Ontogeny in Scirpus, Eriophorum and Dulichium (Cyperaceae), with Special Reference to the Perianth Naturalis
Vrijdaghs, A.; Caris, P.; Goetghebeur, P.; Smets, E..
Background and Aims Based on molecular phylogenetic analysis, it has been suggested recently that the Cyperaceae comprises only two subfamilies: the Mapanioideae and the Cyperoideae. In most flowers of the Cyperoideae, the whorl of inner stamens is reduced, resulting in tetracyclic flowers. In the more primitive (scirpoid) genera within the Cyperoideae, the perianth consists of two polysymmetric whorls, whereas the perianth parts in the more derived genera have been subject to modifications and/or reduction. Comparative studies of the many silky hairs of Eriophorum and of the eight bristles of Dulichium have given rise to much discussion about their homology. Methods The spikelet and floral ontogeny in freshly collected inflorescences was investigated...
Tipo: Article / Letter to the editor Palavras-chave: Dulichium arundinaceum (L.) Britton; Eriophorum latifolium Hoppe; Floral ontogeny; Perianth; Scirpoid flower; Scirpus sylvaticus L; Scanning electron microscopy.
Ano: 2005 URL: http://www.repository.naturalis.nl/record/407290
Registros recuperados: 59
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